I. Nhanganyaya
Phospholipids ikirasi ye lipids iyo yakakosha zvikamu zve cell membranes. Chimiro chavo chakasiyana, chinosanganisira musoro we hydrophilic uye miswe miviri ye hydrophobic, inobvumira phospholipids kuti iite chimiro chebilayer, chinoshanda sechipingamupinyi chinoparadzanisa zviri mukati mesero kubva kune imwe nzvimbo yekunze. Iri basa remaumbirwo rakakosha pakuchengetedza kutendeseka uye kushanda kwemasero mune zvese zvipenyu.
Kuratidzira kwesero uye kutaurirana maitiro akakosha anogonesa maseru kudyidzana pamwe nekwaanogara, zvichibvumira mhinduro dzakarongeka kune dzakasiyana siyana. Masero anogona kudzora kukura, kukura, uye akawanda mabasa emuviri kuburikidza nemaitiro aya. Nzira dzekusaina masero dzinosanganisira kutapurirana kwezviratidzo, senge mahormone kana neurotransmitters, ayo anocherechedzwa nema receptors ari pa membrane yesero, zvichikonzera kuwanda kwezviitiko izvo zvinozotungamira kune chaiyo cellular mhinduro.
Kunzwisisa basa rephospholipids mukusaina masero uye kutaurirana kwakakosha pakuburitsa kuomarara kwematauriro anoita masero uye kuronga mabasa awo. Kunzwisisa uku kune zvinosvika kure mundima dzakasiyana siyana, kusanganisira cell biology, pharmacology, uye kuvandudza kwakanangwa marapirwo ezvirwere zvakawanda uye kusagadzikana. Nekunyatsoongorora kupindirana kwakaomarara pakati pephospholipids uye kusaina masero, tinogona kuwana nzwisiso mumatanho akakosha anotonga hunhu hwemaserura uye basa.
II. Chimiro chePhospholipids
A. Tsanangudzo yePhospholipid Structure:
Phospholipids maamphipathic molecules, zvichireva kuti ane ese ari maviri hydrophilic (inokwezva mvura) uye hydrophobic (inodzoreredza mvura) matunhu. Iyo yakakosha chimiro chephospholipid ine glycerol molecule inosungirirwa kune maviri fatty acid cheni uye phosphate-ine musoro boka. Iyo hydrophobic miswe, inoumbwa nemafuta acid maketani, inoumba mukati meiyo lipid bilayer, nepo hydrophilic misoro mapoka anobatana nemvura pane ese emukati uye kunze kwemukati me membrane. Uku kurongeka kwakasiyana kunobvumira maphospholipids kuti azviunganidze ega mubilayer, aine hydrophobic miswe yakanangana nemukati uye iyo hydrophilic misoro yakatarisana nenzvimbo dzine mvura mukati nekunze kwesero.
B. Basa rePhospholipid Bilayer muSero Membrane:
Iyo phospholipid bilayer chinhu chakakosha chechimiro chesero membrane, ichipa semi-permeable barriers inodzora kuyerera kwezvinhu kupinda nekubuda musero. Kusarudzika uku kwakakosha pakuchengetedza nharaunda yemukati mesero uye kwakakosha kune maitiro akadai sekutora zvinovaka muviri, kubvisa tsvina, uye kudzivirira kubva kune zvinokuvadza. Kupfuura basa rayo rekuumbwa, iyo phospholipid bilayer inoitawo basa rakakosha mukusaina sero uye kutaurirana.
Iyo fluid mosaic modhi yesero membrane, yakakurudzirwa naSinger naNicolson muna 1972, inosimbisa simba uye heterogeneous hunhu hwe membrane, ine phospholipids inogara ichifamba uye akasiyana mapuroteni akapararira mukati me lipid bilayer. Ichi chine simba chimiro chakakosha mukufambisa kusaina kwesero nekutaurirana. Mareceptors, ion channels, uye mamwe maprotein echiratidzo akaiswa mukati me phospholipid bilayer uye akakosha pakuziva masaini ekunze uye nekuaendesa mukati mesero.
Uyezve, izvo zvemuviri zvephospholipids, senge kuyerera kwavo uye kugona kuumba lipid rafts, zvinopesvedzera kurongeka uye kushanda kwemembrane proteins inobatanidzwa mukusaina sero. Hunhu hune simba hwephospholipids hunokanganisa kugariswa uye kuita kwekusaina mapuroteni, nokudaro zvichikanganisa humbowo uye kugona kwemasaini nzira.
Kunzwisisa hukama pakati pephospholipids uye chimiro uye basa re membrane yesero kune zvakakomba kune akawanda biological maitiro, kusanganisira cellular homeostasis, budiriro, uye chirwere. Iko kubatanidzwa kwephospholipid biology pamwe nesero kusaina tsvakiridzo inoenderera mberi nekufumura yakakosha nzwisiso mukuomesesa kwesero kutaurirana uye ine vimbiso yekuvandudzwa kwemaitiro matsva ekurapa.
III. Basa rePhospholipids muSero Signaling
A. Phospholipids seChiratidzo cheMamorekuru
Phospholipids, senhengo dzakatanhamara dzemasero membranes, dzakabuda seakakosha ekusaina mamorekuru mukutaurirana kwesero. Iwo hydrophilic misoro mapoka e phospholipids, kunyanya ayo ane inositol phosphates, anoshanda seakakosha echipiri vatumwa munzira dzakasiyana dzekusaina. Semuyenzaniso, phosphatidylinositol 4,5-bisphosphate (PIP2) inoshanda semamorekuru echiratidzo nekunamirwa muinositol trisphosphate (IP3) uye diacylglycerol (DAG) mukupindura kune extracellular stimuli. Aya mamolekemu anoburitsa lipid anoita basa rakakosha mukudzora intracellular calcium mazinga uye activate protein kinase C, nokudaro modulating akasiyana siyana cellular maitiro anosanganisira kuwanda kwesero, kusiyanisa, uye kutama.
Uyezve, phospholipids senge phosphatidic acid (PA) uye lysophospholipids zvakaonekwa sechiratidzo chemamorekuru anopesvedzera zvakananga mhinduro dzeserura kuburikidza nekudyidzana nekwakananga mapuroteni. Semuenzaniso, PA inoshanda semurevereri akakosha mukukura kwesero uye kuwanda nekuita mapuroteni echiratidzo, nepo lysophosphatidic acid (LPA) inobatanidzwa mukutonga kwe cytoskeletal dynamics, kupona kwesero, uye kutama. Aya mabasa akasiyana-siyana e phospholipids anosimbisa kukosha kwavo mukuronga kwakaomesesa kusaina macascades mukati memasero.
B. Kubatanidzwa kwePhospholipids muSignal Transduction Pathways
Kubatanidzwa kwephospholipids munzira dzekuchinja chiratidzo kunoratidzwa nebasa rayo rakakosha mukugadzirisa chiitiko che membrane-bound receptors, kunyanya G protein-coupled receptors (GPCRs). Pamusoro pe ligand inosunga kuGPCRs, phospholipase C (PLC) inoshandiswa, zvichiita kuti hydrolysis yePIP2 uye chizvarwa che IP3 uye DAG. IP3 inokonzeresa kuburitswa kwecalcium kubva muzvitoro zvemukati, nepo DAG inobata protein kinase C, inozoguma mukugadziriswa kweiyo gene kutaura, kukura kwesero, uye synaptic kutapurirana.
Uyezve, phosphoinositides, kirasi yephospholipids, inoshanda senzvimbo dzekuisa mapuroteni anosanganisirwa munzira dzakasiyana siyana, kusanganisira ayo anodzora membrane kutengesa uye actin cytoskeleton dynamics. Kudyidzana kwakasimba pakati pephosphoinositides uye mapuroteni avo anopindirana anobatsira kumamiriro ekunze uye epanguva yezviitiko zvekusaina, nekudaro achigadzira mhinduro dzemaserura kune extracellular stimuli.
Kubatanidzwa kwakasiyana-siyana kwephospholipids muchitokisi chiratidzo uye nzira dzekushandura zviratidzo zvinosimbisa kukosha kwavo sevatongi vakuru ve cellular homeostasis uye basa.
IV. Phospholipids uye Intracellular Kukurukurirana
A. Phospholipids muIntracellular Signaling
Phospholipids, kirasi ye lipids ine phosphate boka, inotamba mabasa akakosha mukusaina intracellular, kuronga maitiro akasiyana-siyana emasero kuburikidza nekubatanidzwa kwavo mukusaina macascades. Mumwe muenzaniso wakakurumbira phosphatidylinositol 4,5-bisphosphate (PIP2), phospholipid iri mu plasma membrane. Mukupindura kune extracellular stimuli, PIP2 inonamirwa mu inositol trisphosphate (IP3) uye diacylglycerol (DAG) ne enzyme phospholipase C (PLC). IP3 inokonzeresa kuburitswa kwecalcium kubva muzvitoro zvemukati, nepo DAG inobata protein kinase C, inopedzisira yagadzirisa mabasa akasiyana-siyana emasero akadai sekuwedzera kwesero, kusiyanisa, uye cytoskeletal reorganization.
Pamusoro pezvo, mamwe maphospholipids, anosanganisira phosphatidic acid (PA) uye lysophospholipids, akaonekwa seakakosha mukusaina intracellular. PA inobatsira kudzora kukura kwesero uye kuwanda nekuita se activator yemapuroteni akasiyana-siyana echiratidzo. Lysophosphatidic acid (LPA) yave ichizivikanwa nekubatanidzwa kwayo mukugadzirisa kwesero kupona, kutama, uye cytoskeletal dynamics. Izvi zvakawanikwa zvinosimbisa akasiyana uye akakosha mabasa ephospholipids sechiratidzo mamorekuru mukati mesero.
B. Kudyidzana kwePhospholipids neProteins uye Receptors
Phospholipids zvakare inopindirana nemapuroteni akasiyana-siyana uye zvinogamuchirwa kuti zvigadzirise nzira dzechiratidzo chemasero. Zvinonyanya kukosha, phosphoinositides, boka diki rephospholipids, inoshanda semapuratifomu ekutora uye activation yekusaina mapuroteni. Semuenzaniso, phosphatidylinositol 3,4,5-trisphosphate (PIP3) inoshanda seyakakosha kudzora kwekukura kwesero uye kuwanda nekutsvaga mapuroteni ane pleckstrin homology (PH) domains kune plasma membrane, zvichibva zvatanga zviitiko zvekudzika pasi. Uyezve, kushamwaridzana kune simba kwephospholipids nekuisa mapuroteni uye zvinogamuchirwa zvinobvumira kunyatsogadzirisa spatiotemporal yezviratidzo zvezviratidzo mukati mesero.
Iko kupindirana kwakasiyana-siyana kwephospholipids nemapuroteni uye zvinogamuchira zvinoratidzira basa ravo rakakosha mukugadziriswa kweiyo intracellular siginecha nzira, pakupedzisira zvichibatsira kukutonga kwesero mabasa.
V. Mutemo wePhospholipids muSero Signaling
A. Enzymes uye Nzira Dzinobatanidzwa muPhospholipid Metabolism
Phospholipids inodzorwa zvine simba kuburikidza neyakaomesesa network yema enzymes uye nzira, ichipesvedzera kuwanda kwavo uye basa mukusaina sero. Imwe nzira yakadai inosanganisira synthesis uye shanduko yephosphatidylinositol (PI) uye yayo phosphorylated derivatives, inozivikanwa se phosphoinositides. Phosphatidylinositol 4-kinases uye phosphatidylinositol 4-phosphate 5-kinases ndiwo ma enzymes anokonzera phosphorylation yePI panzvimbo yeD4 uye D5, inogadzira phosphatidylinositol 4-phosphate (PI4P) uye phosphatidylinosi-phosphate (Plytobi2), IPlyitol-phosphate (Plytobi2). Sezvineiwo, phosphatase, senge phosphatase uye tensin homolog (PTEN), dephosphorylate phosphoinositides, kudzora mazinga avo uye kukanganisa kweserura kusaina.
Uyezve, iyo de novo synthesis ye phospholipids, kunyanya phosphatidic acid (PA), inopindirana nema enzymes akaita se phospholipase D uye diacylglycerol kinase, apo kuora kwavo kunokonzerwa ne phospholipases, kusanganisira phospholipase A2 uye phospholipase C. Aya maitiro enzymatic ekugadzirisa pamwe chete kutonga pamwe chete. bioactive lipid mediators, inokanganisa akasiyana maseru kusaina maitiro uye nekubatsira mukuchengetedza cellular homeostasis.
B. Mhedzisiro yePhospholipid Regulation pane Cell Signaling Processes
Kudzora kwephospholipids kune mhedzisiro yakadzika pamaseru ekusaina maitiro nekugadzirisa zviitiko zvakakosha zvekusaina mamorekuru uye nzira. Semuenzaniso, kutengeswa kwePIP2 nephospholipase C inogadzira inositol trisphosphate (IP3) uye diacylglycerol (DAG), zvichiita kuti kusunungurwa kwe intracellular calcium uye kushandiswa kweprotein kinase C, maererano. Iyi inosaina cascade inopesvedzera mhinduro dzemaserura senge neurotransmission, kutsikisa tsandanyama, uye immune cell activation.
Uyezve, shanduko mumazinga ephosphoinositides inokanganisa kutorwa uye kushandiswa kweanoita mapuroteni ane lipid-binding domains, anokanganisa maitiro senge endocytosis, cytoskeletal dynamics, uye cell migration. Pamusoro pezvo, kudzorwa kwemazinga ePA nephospholipases uye phosphatases kunokanganisa kutengesa kwe membrane, kukura kwesero, uye lipid kusaina nzira.
Kudyidzana pakati pephospholipid metabolism uye sero signing inosimbisa kukosha kweiyo phospholipid mutemo mukuchengetedza cellular basa uye kupindura kune extracellular stimuli.
VI. Mhedziso
A. Pfupiso Yemabasa Akakosha ePhospholipids muSero Signaling uye Kukurukurirana
Muchidimbu, phospholipids inotamba mabasa akakosha mukuronga masero ekusaina uye maitiro ekutaurirana mukati mebiological system. Kusiyana kwavo kwemaitiro uye kushanda kunoita kuti vakwanise kushanda sevanochinja-chinja mhinduro dzemaserura, vaine mabasa akakosha anosanganisira:
Membrane Organisation:
Phospholipids inoumba zvivharo zvakakosha zvemaserura membranes, inomisikidza hurongwa hwekupatsanurwa kwezvikamu zvemasero uye nekumisikidzwa kwemapuroteni echiratidzo. Kugona kwavo kugadzira lipid microdomain, senge lipid rafts, inopesvedzera kurongeka kwenzvimbo yekusaina macomplexes uye kudyidzana kwawo, zvichikanganisa kusaina chaiko uye kugona.
Signal Transduction:
Phospholipids inoita sevamiriri vakakosha mukufambiswa kwemasaini ekunze kune intracellular mhinduro. Phosphoinositides inoshanda semamolekemu anosaina, achigadzirisa zviitiko zveakasiyana siyana anoita mapuroteni, nepo emahara mafuta acids uye lysophospholipids anoshanda seyechipiri vatumwa, achipesvedzera activation yekusaina cascades uye gene kutaura.
Kuchinja Masaini Sero:
Phospholipids inobatsira kudzora nzira dzakasiyana dzekusaina, kuita kutonga pamusoro pemaitiro akadai sekuwedzera kwesero, kusiyanisa, apoptosis, uye mhinduro dzemuviri. Kubatanidzwa kwavo mukugadzirwa kwebioactive lipid mediators, kusanganisira eicosanoids uye sphingolipids, kunowedzera kuratidza maitiro avo pakuzvimba, metabolic, uye apoptotic signing network.
Intercellular Communication:
Phospholipids inobatanidzwawo mukukurukurirana kwe intercellular kuburikidza nekubudiswa kwe lipid mediators, zvakadai se prostaglandins uye leukotrienes, iyo inogadzirisa mabasa emasero ekuvakidzana uye matishu, kugadzirisa kuzvimba, kuona kurwadziwa, uye vascular function.
Mipiro yakasiyana-siyana ye phospholipids kune kusaina masero uye kutaurirana kunosimbisa kukosha kwavo mukuchengetedza cellular homeostasis uye kuronga mhinduro dzemuviri.
B. Nhungamiro Yenguva Yemberi Yekutsvakurudza pamusoro pePhospholipids muCellular Signaling
Sezvo mabasa akaomarara e phospholipids mukusaina sero achiramba achivhurwa, nzira dzinoverengeka dzinonakidza dzetsvagurudzo yeramangwana dzinobuda, dzinosanganisira:
Interdisciplinary Approaches:
Kubatanidzwa kwehunyanzvi hwekuongorora maitiro, senge lipidomics, ine mamorekuru uye cellular biology inosimudzira manzwisisiro edu emamiriro ekunze uye epanguva ye phospholipids mumasaini maitiro. Kuongorora iyo crosstalk pakati pe lipid metabolism, kutengeswa kwemembrane, uye kusaina kwemaserura kuchaburitsa nzira dzinoverengeka dzekudzora uye zvinangwa zvekurapa.
Maonero eSystem Biology:
Leveraging systems biology nzira, kusanganisira masvomhu modelling uye network ongororo, zvinozogonesa kujekeswa kwekukanganisa kwepasi rose kwephospholipids pamaserura ekusaina network. Kuenzanisira kupindirana pakati pe phospholipids, ma enzymes, uye masigning anoita zvinojekesa zvivakwa zvinobuda uye nzira dzemhinduro dzinotonga kusaina nzira yegwara.
Therapeutic Zvinoreva:
Kuongorora dysregulation ye phospholipids muzvirwere, senge kenza, neurodegenerative kusagadzikana, uye metabolic syndromes, inopa mukana wekuvandudza kurapa kwakanangwa. Kunzwisisa mabasa ephospholipids mukufambira mberi kwechirwere uye kuona nzira nyowani dzekugadzirisa zviitiko zvavo zvine chivimbiso chehunyanzvi hwekurapa nzira.
Mukupedzisa, ruzivo runoramba ruchiwedzera rwe phospholipids uye kubatanidzwa kwavo kwakaoma mukusaina cellular uye kutaurirana kunopa muganhu unonakidza wekuenderera mberi nekuongorora uye inogona kushandura shandurudzo mundima dzakasiyana dze biomedical research.
References:
Balla, T. (2013). Phosphoinositides: diki lipids ine giant kukanganisa pane sero regulation. Physiological Ongororo, 93(3), 1019-1137.
Di Paolo, G., & De Camilli, P. (2006). Phosphoinositides mune cell regulation uye membrane dynamics. Zvisikwa, 443(7112), 651-657.
Kooijman, EE, & Testerink, C. (2010). Phosphatidic acid: inobuda yakakosha mutambi mukusaina sero. Maitiro muSainzi Yekudyara, 15 (6), 213-220.
Hilgemann, DW, & Bhora, R. (1996). Kudzora kwemoyo Na (+), H (+) -exchange uye K (ATP) potassium nzira nePIP2. Sayenzi, 273(5277), 956-959.
Kaksonen, M., & Roux, A. (2018). Nzira dze clathrin-mediated endocytosis. Masikirwo Ongororo Molecular Cell Biology, 19(5), 313-326.
Balla, T. (2013). Phosphoinositides: diki lipids ine giant kukanganisa pane sero regulation. Physiological Ongororo, 93(3), 1019-1137.
Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2014). Molecular Biology yeSero (6th ed.). Garland Sayenzi.
Simons, K., & Vaz, WL (2004). Model masisitimu, lipid rafts, uye cell membranes. Ongororo yegore negore yeBiophysics uye Biomolecular Structure, 33, 269-295.
Nguva yekutumira: Zvita-29-2023
