Nhau - Natural Villin Kugadzirwa Kubva Kune Renewable Zviwanikwa

I.iproduction

VIILILIN ndeimwe yeanonyanya kufarirwa uye yakashandiswa zvakanyanya flavor compounds munyika. Netsika, yakabviswa kubva kune vanilla bhinzi, iyo inodhura uye inosangana nematambudziko nezvekusimba uye kupa ketani yekumanikidza. Nekudaro, nekufambira mberi muBiotechnology, kunyanya mumunda wezvemicrobial biotransformation, imwe nguva itsva yechisiko meillin kugadzirwa kwakabuda. Kushandisa mikana yehutachiona kweiyo biology yekushanduka kwezvinhu zvakasikwa zvinhu zvakapa nzira yehupfumi inobatsira yehupfumi yeiyo synthesis yeVanillin. Iyi nzira isingori kero chete yekumanikidza kunetsekana asi zvakare inopa mhinduro dzesviro yeiyo flavour indasitiri yeflavo. Tsvagurudzo inoitiswa neSerm Institute yeSainzi uye tekinoroji (Srmist) yakavezwa nzira dzeBiological yeillhesis yeBiological yeillhesis kubva kune akasiyana subsin

Ii. Maitiro Ekuwana Natural Veillin Kubva Kune Renewable Zviwanikwa

Kushandiswa kweiyo ferlic acid seye substrate

Ferulic acid, inotorwa kubva kune sosi kunge mupunga macroc uye oat branes kufanana naVanillin uye anoshandiswa seyakajeka precursor substrate yeillin kugadzirwa. Hutano dzakasiyana-siyana dzakadai saPseudomson, Aspergillus, hutachiona, uye fungi dzakashandiswa pakugadzirwa kweillin kubva kune ferlic acid. Zvine musoro, marudzi akadai saAmycolatopsis uye chena-rot-rot fungi akaonekwa sevanogona kuvhomhowa kuti vabudise veillin kubva kune ferlic acid. Zvidzidzo zvakati wandei zvakaongorora kugadzirwa kweViillin kubva kune ferdic acid uchishandisa hutachiona, nzira dzenzombe, uye dzisimbe, zvichiratidza kuwanda uye kugona kwenzira iyi.

Enzymatic Syntymatic yeVanillin kubva kuFerlic Acid inosanganisira iyo kiyi yeNzyme Feruloyslslslsls, iyo inobata hydrolysis yesangano muAxter acid, kusunungura vanchidin uye zvimwe zvinoenderana ne-zvigadzirwa. Nekuongorora huwandu hwakaringana hweVillin Biosynthetic enzymes muCell-emahara masisitimu eSecerichica coli Pamusoro pezvo, kushandiswa kweiyo microbili cell yekusahadzika kwakaparara nekuda kweiyo yakanakira biocomputer uye kugadzikana pasi pemamiriro akasiyana siyana. Nyanzvi yekusaita isingaiti yeNewlibilation yeVillin kugadzirwa kubva kuServic Acid yakagadzirwa, kubvisa kudiwa kweCoenymes. Iyi nzira inosanganisira coenyme-yakazvimirira decarboxylase uye coenyme-yakazvimirira oxygenase ine mhosva yekutendeuka kwekutendeuka kwaiid. Iyo CO-ILEBILIZE yeFDC uye CSO2 inoita kuti kugadzirwa kwe2,5 mg yeVillin kubva kuChillin Acces

Kushandiswa kweEugel / isoeugol seSubstrate

Eugel naiyoOeugol, paanosarudzika, pakatarisana neBioconvers, gadzira vaillin uye zvinoenderana neyakaenderana ne metabolites, iyo yakawanikwa kuti ive neyakasiyana-siyana mafaro uye kukosha kwakakosha kwehupfumi. Zvidzidzo zvakati wandei zvakanyatsoongorora kushandiswa kwezvinhu zvakagadziridzwa uye zvakasarudzika zvinoitika microorganisms kuSynthessizeni kubva kuEugol. Iko kugona kwekusvibiswa kweEugenic kwakaonekwa mumabhakitiriya akasiyana siyana uye fungi, kusanganisira asi vasingabatanidzwe naBacillus, pseudomonas, asperokollus, uye vaPhodocccus, vachiratidzira kugona kwavo muEugellin kugadzirwa. Iko kushandisa kweEugenol oxidase (eugo) se enzyme kugadzirwa kweillin munzvimbo yeindasitiri yakaratidza kukosha kwakakura. Eugo inoratidza kugadzikana uye chiitiko pamusoro penzvimbo yakafara pH, ine svutugadzike yekuwedzera inowedzera chiitiko uye kuderedza nguva yekuita. Uyezve, kushandiswa kweiyo lexilized eugo inobvumira kudzoreredzwa kweiyo biocatalyst mukusvika kune 18 reaction cleaces, inotungamira kune inopfuura gumi nemaviri-folding kuwedzera kwe biocatalyst goho reil. Saizvozvo, fungisiki enzyme cso2 inogona kusimudzira kushandurwa kweSoeugol muVoeegol muVanillin pasina kuvimba neCenymes.

Dzimwe Substrates

Pamusoro peiyo ferlic acid uye eugenol, mamwe makomendi akadai saVarillic acid uye C6-c3 phenylpropanoids akaonekwa sevanogona kugadzirwa kweillin kugadzirwa. Vanillic acid, yakagadzirwa seyakagadzirwa neyakagadzirwa nekurohwa kana sechidimbu kukwikwidza mu metabolic nzira, inoonekwa seyakakosha precursor ye bio-bosmillin kugadzirwa. Uyezve, kupa ruzivo rwekushandiswa kweC6-c3 phenylpropanoids yeVillin synthesis inopa mukana wakasarudzika wekutsigira uye hunyanzvi hwekuvandudza hunyanzvi.

Mukupedzisa, kushandiswa kwekuvandudza zviwanikwa zvemusikirwo edillin kugadzirwa kuburikidza ne microbial biotransformation is a landmark budiriro muindasitiri yeflavor. Iyi nzira inopa imwe nzira, yakasimba nzira yekugadzirwa kweVillin, kugadzirisa zvinonetsa uye kuderedza kuvimba nenzira dzechinyakare nzira dzekutsamwa. Zvinoshanda zvakasiyana uye kukosha kwehupfumi kweViillin kuyambuka indasitiri yechikafu inosimbisa kukosha kwekuenderera mberi kwekutsvaga nekuvandudza munzvimbo ino. Kufambira mberi kwemberi mumunda wezvakasikwa meillin kugadzirwa kune mukana wekuchinja iyo indasitiri yekurima, inopa nzvimbo dzakasimba uye dzeco-fundo dzimwe nzira dzekunyepedzera. Sezvo isu tichiramba tichisaririra kugona kwekuvandudza zviwanikwa uye kufambira mberi kwekutanga kwekutanga, kugadzirwa kwechikepe chechillin kubva kune dzakasiyana-siyana dzinovimbisa mari yekuvimbisa yekuvandudza flavour hunyanzvi.

III. Ndezvipi zvakanakira kushandisa zvinowanikwa zviwanikwa kuburitsa zvakasikwa meillin

Zvakatipoteredza Hushamwari:Kushandisa zvinowanikwa zviwanikwa zvakadai sezvirimwa uye biomass marara kuti zvibudise vakillin zvinogona kuderedza kudiwa kweiyo fossil mafuta, kuderedza kukanganisa kwakashata pane zvakatipoteredza, uye kuderedza greenhouse gasi.

Sustainabilation:Kuishandisa zviwanikwa zvekuvandudza kunogonesa kuwanikwa kwesimba uye zvishandiso zvekushandisa, kubatsira kuchengetedza zviwanikwa uye kuzadzisa izvo zvinodiwa nezvizvarwa zvinotevera.

Dziviriro yePiotiity:Kuburikidza nekushandiswa kwekunyepedzera kwezvinhu zvinotsigirwa, zviwanikwa zvesango zvinowanikwa zvinogona kudzivirirwa, izvo zvinobatsira kuchengetedzwa kwezvinhu zvipenyu uye kuchengetedza kwezvinhu zvakasikwa.

Mhando yechigadzirwa:Kuenzaniswa neSynthetic Vanillin, Villation Villin vanogona kunge vaine zvakanakira mune hunhu hunhu uye hunhu hwechisikigo, izvo zvichabatsira kuvandudza kunaka uye kunhuhwirira kwezvinhu zvinonakidza.

Kuderedza kutsamira pane fossil mafuta:Iko kushandiswa kwezvinhu zvinotsigirwa kunobatsira kuderedza kuparadzira kushomeka kwemafusita mafuta, ayo anobatsira pakuchengetedza simba uye simba rekusiyana siyana. Ndinovimba ruzivo rwuri pamusoro runogona kupindura mibvunzo yako. Kana iwe uchida gwaro rezita muChirungu, ndokumbirawo undizivise kuti ndigokupa iwe.

Iv. Mhedziso

Iko kugona kwekushandisa zviwanikwa zvekuvandudza kuburitsa zvakasikwa veillin sechinhu chakasimba uye zvakatipoteredza hushamwari mune imwe nzira yakakosha. Iyi nzira inobata vimbiso mukugadzirisa kuwedzerwa kwekuda kweVataillin vechillin vachiri kuderedza kuvimba kwemaitiro ekugadzira.

Inillin zvisikwa zvinokonzeresa nzvimbo yakakosha muindasitiri yeflavor, inokosheswa nehunhu hwechiratidzo uye inoshandiswa pakushandiswa kwakazara senge mumiririri anotsvaga muzvigadzirwa zvakasiyana siyana. Izvo zvakakosha kusimbisa kukosha kweyechisiko meillin seyakatsvakirwa-mushure mekunyepedzerwa mune chikafu, chinwiwa, uye maindasitiri ekunhuhwirira nekuda kwekunyepedzera kwepamoyo uye madhiri emutengi kune zvakasikwa flavors.

Uyezve, iyo munda wezvisikwa veillin kugadzirwa kunopa mikana yakakura yekuwedzera kutsvagisa nekuvandudza. Izvi zvinosanganisira kuongorora matekinoroji matsva uye nzira dzekunyevesa dzekusimudzira kushanda uye kusimudzira kwekubereka midzitiro zvakasikwa kubva kune zvinowanikwa zviwanikwa. Pamusoro pezvo, kukudziridzwa kwekutyisa uye kwakabudirira-kuita nzira dzekugadzira dzinotora basa rekusimudzira mukutsikisa kuyamwiswa kweItillin yeIllin seyakagadziriswa uye eco-johiss mune indasitiri yeflavor.